FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_81_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_81_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774637
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT64420.36300381430117823No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCT52760.2973002366117691No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA45540.25661586003222064No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT32470.18296699550386925No Hit
CATAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAA30920.17423281493623766No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC30130.16978120032434804No Hit
TAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAACA27980.1576660466337623No Hit
GGCTTGGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGA26570.14972075979482No Hit
GGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGAAAATA26440.14898821561817993No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA26260.1479739236812937No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG26200.14763582636899827No Hit
TGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTGTGAAGAAATATCTA25180.1418881720599762No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCCTCATTGTTTCTTTGGC23840.1343373320853786No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC23660.13332304014849233No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG22900.1290404741927504No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC22710.12796983270381493No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA21720.12239122705094056No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT21540.1213769351140543No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG21470.12098248824970967No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG21310.12008089541692189No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG20910.11782691333495243No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT20820.11731976736650933No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC20030.11286815275461967No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT19900.1121356085779796No Hit
CCTCCATGTTGTCAGAGAGTACTTCCCTCATTGTTTCTTTGGCTTGGATT19370.1091490823193701No Hit
GGTCAGTACAGTGGGTTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA19220.10830383903863157No Hit
GTCAGAGAGTACTTCCCTCATTGTTTCTTTGGCTTGGATTTCTTTTTGGT18860.10627525516485907No Hit
AAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATCCAAG18840.1061625560607606No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA18510.10430302084313581No Hit
GTGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTGTGAAGAAATATCT18160.10233078652141253No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGT100.0070910337145.00197145
CGTCAGA100.007091631144.997882
TACCGAT259.3884976E-486.998737
CAACGCA11800.082.329319
GTAACAA14000.076.1238864
AACAACG14600.067.036697
ACAACGC15650.062.539028
TAACAAG18900.056.3880655
AACACGT4000.056.186686
AGAAGCG3150.055.2372935
GAGCAGA8150.055.1791
ATACCGA400.00605200554.3742076
AAACACG12300.053.637435
TAGTAAC21000.053.5111242
GAAGCGG5050.053.143491
CACGAGC8350.052.095058
GAAACAC13050.050.554824
CGGAGCG752.5516092E-548.332635
AGTAACA23750.047.0098423
ACACGTG4800.046.8222357
Produced by FastQC (version 0.11.5)