FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_80_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_80_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092946
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA10970.10037092408957077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA15600.086.0239261
AACACGT4750.085.4728246
TAGTAAC15750.082.86012
AGTAACA17950.072.701223
AAACACG14400.063.9403275
GCCGGCG350.00356608962.145072
ACAACGC4850.059.7932138
GAAACAC15550.059.211624
GTAACAA21950.058.461824
AACACGA9700.055.308726
AACAACG5350.054.2050637
TAGAAAC24400.053.7826542
GTAGAAA24700.053.4498561
CACGAGC10400.051.5860188
AGAAGCG6250.051.0394865
ACACGAG10550.050.852577
ATTAGTA7650.050.255841
CAACGCA6800.050.1097879
CGGAGCG450.00963975948.3328485
ACACGTG9200.044.9180227
Produced by FastQC (version 0.11.5)