FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_78_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_78_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235866
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA31940.25844225830308465No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22770.18424327556547393No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA20840.168626695774461No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC17360.14046830319791953No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAATCTCAGTAAGAACAATT16150.13067759773308757No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA16130.13051576789069363No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG14620.11829761478995295No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG13770.1114198464882115No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG12910.10446116326527309No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA151.2301309E-4144.998343
GCACGCA13000.0105.4069062
AGCACGC13800.098.822961
TAGTAAC36550.097.595172
CGAGCGA451.0919393E-896.665565
GTAACAA37350.095.5009164
GTAGTAA39100.091.649331
CGCACTT15650.089.871185
CACGCAC15550.088.117963
AGTAACA42700.083.705133
TAACAAG43500.082.1657265
AAACACG16100.081.955585
ACGCACT17300.081.299654
GAAGCGG4000.074.353771
CGGGTAC1300.072.505035145
ACGAGCG702.8139766E-972.499174
GAAACAC19200.069.100774
CGGCGGC1250.063.799279
ACGTGCA6850.063.505492
CAAGAGC52600.062.5753338
Produced by FastQC (version 0.11.5)