FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_77_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_77_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557153
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT84120.5402166646437441No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC49860.32019974915759725No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT43500.279355978506929No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA35140.2256682548214594No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG32480.20858579728517368No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA28370.1821914737986569No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC24360.15643934796388023No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT23530.15110910745443767No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT23310.14969627262060953No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA21390.13736607770720025No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC20990.13479728710024No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT18870.12118269688335057No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG18490.11874234580673833No Hit
GTAAGAAGTACAGTAAAAATGAATTTAATGCGATTTTTCTACCTCCAAAA18220.11700841214704015No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT17190.11039377633411745No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC17160.11020111703859545No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT17010.10923782056098533No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGCTAGCGTTAGGGCA16810.1079534252575052No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT16680.1071185683102431No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC16490.105898392771937No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG16400.10532041488537094No Hit
GGCTTGGGCTGTCCCAAAGGACAACAACAAAAATGCAACGAACCCATTAA16220.1041644591122388No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA16160.10377914052119477No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA15880.10198098709632258No Hit
GTAATAAAAGGGTCCTTGCCTTTAATTGGTGAAGCAGATTGCCTTCATGA15660.10056815226249444No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGT100.0070896656145.01076145
AGCACGC12450.0131.092761
GCACGCA12600.0129.461432
CACGCAC12800.0127.438583
GAAACAC8750.0124.282974
AAACACG9350.0116.307595
CGCACTT17000.098.512525
ACGCACT17050.098.223634
CGGTGCA301.5999163E-596.664532
GTAACAA29550.093.475094
GAGCACG259.3682203E-487.045591
AACACGA11200.086.091846
AACACGT1500.082.164846
CAACGCA4400.080.736859
GCGGTGC451.2293749E-680.597771
AGTGGGT554.3966793E-879.089165
ACAACGC4500.078.9426968
ACGTGCA2850.078.85792
TAACAAG36800.075.059485
ACACGAG12900.074.184417
Produced by FastQC (version 0.11.5)