FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_75_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_75_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178026
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT23090.10601342683696155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA30150.090.235421
AGCACGC19950.085.09541
TAGTAAC31850.084.6773762
ACGCACT21950.078.9400254
GCACGCA22350.076.554082
CGCACTT16500.075.135655
TAACAAG38850.068.3005145
AGTAACA41150.065.187713
GTAACAA42600.062.458334
CACGCAC30800.055.7868083
AAACACG23800.052.0898445
GCGCATA701.6967464E-551.785222
ACACGAG16550.046.872677
GTAGAAA35000.045.395121
CGCACTG5950.045.0836075
CAAGAGC58350.044.4811558
AACACGT10400.043.9178546
AACACGA18600.042.4861566
CGCACTA2950.041.7792665
TAGAAAC37250.041.0667232
Produced by FastQC (version 0.11.5)