FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_6_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_6_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190218
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT37500.1712158333097436No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC36130.1649607481994943No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA35390.1615820890888487No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA32700.14930020664609642No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG32250.1472456166463795No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC32110.14660641086868978No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT31730.14487142375781772No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA30780.14053395598063756No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG30620.1398034350918493No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT29230.13345703487050148No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG28240.12893693687112423No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT27470.12542130509383084No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC27280.12455381153839482No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT27100.12373197553850804No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG26700.12190567331653744No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA26030.11884661709473669No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG25710.1173855753171602No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC25480.11633545153952711No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC25130.11473743709530283No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC24530.11199798376234694No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT24450.11163272331795283No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT24420.11149575065130503No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC24000.1095781333182359No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT23590.10770617354071603No Hit
AATGATAACTGATGGCTCAGCTTCAGGTGTTAGTGAATGCAGATTCCTTA23510.10734091309632192No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC23110.10551461087435132No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC22910.10460145976336603No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT22860.1043731719856197No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG21980.10035530709728438No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAA100.0070911446145.00198145
GTAACAA28250.084.432854
TAACAAG33050.072.3896565
AAACACG9150.069.726145
ACACGTG3750.065.732737
TAGTAAC37350.065.418882
AACACGT3800.064.867836
GTCGTCT350.003567199562.1422928
AGTAACA39800.061.5697863
GAAGCGG7850.059.1509551
ACGTGCC1051.1095835E-955.2375959
AACACGA7150.054.7547456
TAGCGTG400.006052063354.37457
AGAAGCG4150.054.1561365
AAGCGGA8750.053.031722
CACGAGC7450.052.5498548
GAAACAC12350.051.072414
CGGAGCG2150.050.5809365
CTATAGG450.00964100448.3328933
GTAGCGT450.00964100448.3328936
Produced by FastQC (version 0.11.5)