FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_68_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_68_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4116551
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA147060.35724080668501373No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC102270.248436130148758No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA96760.23505113868381566No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT69150.16798042827600093No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA68230.16574554766842436No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT67600.16421514029584475No Hit
AGTAGAAACAACGCACTTAGCCGATAAAGAATTTTTTGGCTAGAGGGCTT65120.15819067952759483No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA64350.15632018162777528No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT62830.1526277701891705No Hit
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA61000.14818230115453446No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG59960.14565591438075223No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC58860.1429837745238672No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG57300.13919419436319386No Hit
CCCTTAGACAGTGCCGTTCAATGTTTTCAAATCTTGTTACTATTACTCTT53890.1309105608068502No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC52410.12731531809031396No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC45210.10982494811797545No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG42320.10280450794852292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA83800.081.6266861
TAGTAAC96150.070.804232
ACAACGC38350.066.165228
AACAACG42350.061.285357
CAACGCA64650.054.9483459
AGTAACA126150.054.0790983
GTAACAA128750.052.987014
TAACAAG129050.052.8076555
ACACGAG42250.051.30647
AAACACG65750.051.051955
CACGAGC44950.047.7407348
ACGAGCC19200.047.5769279
ACAAGAG146650.046.519457
CAAGAGC141850.045.027068
AAGAGCC94100.044.762429
AACACGA49600.044.1420366
AAGAGCG14750.042.2701239
ACGAGCA17700.041.7786039
ACGTGCA11300.041.0609139
GTAGAAA152650.040.91391
Produced by FastQC (version 0.11.5)