FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_67_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_67_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235762
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA27930.22601439435748955No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA21240.17187775639645822No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG14840.12008784863104707No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA14770.11952139651486289No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC12350.0102.793521
GCACGCA12500.0102.082482
TAGTAAC33800.098.6710362
GTAGTAA34650.097.561241
CGCACTT15100.089.303195
CACGCAC15250.083.195383
AGTAACA40750.082.017083
GTAACAA40600.081.427254
ACGCACT17150.081.164864
TAACAAG41150.079.986565
AAACACG19500.077.703875
GAAACAC21300.070.796984
AACACGT7250.066.998926
GTCGTAC350.00355836362.179591
ACAAGAG56650.058.229427
AACACGA18350.057.28796
AACAAGA58250.056.7544486
CAAGAGC53950.056.7089968
ACAACGC3850.054.609518
ACGAGCC9200.053.5860869
Produced by FastQC (version 0.11.5)