FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_66_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_66_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093360
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT39540.3616375210360723No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC36870.3372173849418307No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT34100.31188263700885344No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT32760.29962683836979587No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT28450.26020706812028976No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT25570.2338662471647033No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC23520.21511670447062267No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG20940.19151971903124315No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT18320.16755688885636938No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC16180.14798419550742664No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG15500.1417648350040243No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAATCTTCTC15300.139935611326553No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC14870.13600278041998975No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC14550.1330760225360357No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT13960.12767981268749543No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC13850.1266737396648862No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT13790.12612497256164484No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT13730.12557620545840345No Hit
GTTTTCAACTACTAATACAAACCCTCTTATTTGTATTCCAGCAGTGGCAA13700.12530182190678274No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA13460.12310675349381722No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT13210.12082022389697812No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC13020.11908246140338041No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA12880.1178020048291505No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA12870.11771054364527694No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT12850.1175276212775298No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT12850.1175276212775298No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG12540.11469232457744932No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC12360.11304602326772518No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA12330.11277163971610449No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT12310.11258871734835735No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA12030.11002780419989756No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT11980.10957049828052975No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG11630.106369356844955No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA11440.10463159435135727No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT11290.10325967659325383No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT11020.10079022462866759No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA10940.1000585351576791No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG100.0070884586145.01706145
AGCACGC4050.0132.545351
CACGCAC4350.0123.325283
GTAACAA26500.0123.105174
GCACGCA4350.0121.664292
TAACAAG29450.0110.773765
GAAACAC9750.0109.300554
GAGCAGA1950.0104.162451
AAACACG10400.0102.469265
TAGAAAC12550.090.6954352
ACGCACT5950.090.1621864
CGCACTT5950.090.1621865
TAGTAAC37250.088.360692
TCTACTA1150.088.255115
GAAGCGG2550.088.187961
CGGAGCG750.086.9943165
CAGCATC608.2945917E-1084.577814
ACGTGCA4050.082.344062
AGTAACA40050.082.1794053
AACACGT2650.082.0701146
Produced by FastQC (version 0.11.5)