FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_65_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_65_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076983
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT26930.25005037219714704No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC23540.21857355222877242No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT22090.2051100156641284No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA21330.1980532654647288No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG15620.14503478699292374No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA14790.1373280729593689No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC14360.13333543797812963No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT13950.12952850694950616No Hit
CCTTTAATAGTTTTGCAGGAGGTCTATATTTGGTTCCATTGGCAAGCTTC11540.10715118065930473No Hit
AAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACA11140.10343710160698916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC10350.0120.543841
GCACGCA10900.0114.408212
CGCACTT12300.0106.096845
CACGCAC12750.099.509143
ACGCACT13650.098.259414
GTAACAA38050.096.602514
TAGTAAC39900.092.309432
TAACAAG40050.091.235345
AGTAACA44150.083.583813
GTAGTAA45000.082.0469061
AAACACG13750.078.0358355
GAAACAC14250.075.297744
ACAAGAG55900.065.625687
GAAGCGG3250.064.724931
CAAGAGC50300.064.427998
ACGAGCG702.3348184E-762.142449
AACAAGA59400.061.88096
GTCTCGC6150.060.1522671
CGCTCTG6550.058.6637275
TAGAAAC23450.058.4351582
Produced by FastQC (version 0.11.5)