FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_64_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_64_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1342881
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAA46690.34768531239923717No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC45600.3395684353267341No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT44960.3348025625502185No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT35000.26063366746569505No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT29580.22027268238957884No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC27280.203145327098976No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT26170.1948795165022068No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC19260.14342298386826532No Hit
ATTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACA18620.13865711109174975No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT17890.13322103745603667No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC17740.13210403602404086No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC17490.13024236697071445No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG15900.11840215179155861No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA15810.11773195093236109No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT15800.11765748417022805No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC15630.1163915492139661No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA15500.11542348130623636No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC15500.11542348130623636No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA15370.11445541339850664No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG15130.1126682111073133No Hit
ATATCCGAATATAGTAAGTATGATAATGAAGCTGACACATATAGTGATAA14730.10968954062199109No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT14370.10700873718520107No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT14370.10700873718520107No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA14310.10656193661240275No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG14270.10626406956387052No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT14200.10574280222893913No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT14130.10522153489400773No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC14090.10492366784547551No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA14090.10492366784547551No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA13820.102913065267883No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG13550.1009024626902905No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG13520.10067906240389135No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGCG100.007091779144.995568
AGAGCGG100.007091779144.995566
GTAACAA29250.0125.1671144
GAAACAC10000.0119.621344
AGCACGC2400.0114.865141
GCACGCA2450.0112.449732
AAACACG10850.0110.2500845
ACATCGA203.866819E-4108.746679
CACGCAC2700.0102.033923
TAACAAG37350.098.216545
TAGAAAC13050.095.556192
TAGTAAC40850.090.692162
CGCACTT3350.082.236295
AACACGT3000.082.1641546
ACGCACT3450.079.852634
ACGTGCA4500.078.944972
AGTAACA47150.078.8788153
AACACGA12100.078.4893346
ACACGAG12800.074.196957
CTACCCA300.001930979372.54641
Produced by FastQC (version 0.11.5)