FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_63_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_63_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933942
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG17280.18502219623916688No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC16430.17592098866953196No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA13020.13940908536076116No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC11100.11885106355640929No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT10130.10846497962400234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC4450.084.77771
GCACGCA4600.081.956132
AAACACG6850.079.379185
GTAACAA22000.075.795094
AACACGA5100.075.342776
CACGCAC5050.074.653113
TAGTAAC23250.072.031912
TAACAAG24000.070.0835
CGCACTT5750.069.3474965
ACAACGC3200.065.702818
ACGCACT6700.061.6788064
GTAGTAA28700.059.657961
AGTAACA28300.058.9219783
GAAACAC9850.055.938824
ACACGAG6750.053.703457
CAACGCA4950.052.727029
CACGAGC6500.052.422828
AACACGT3500.051.7854656
ACAAGAG33900.049.1885577
AGAAGCG1950.048.33315
Produced by FastQC (version 0.11.5)