FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_61_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_61_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729670
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT21320.29218687900009593No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC11560.15842778242219085No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG10520.14417476393438128No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC8500.11649101648690503No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT8480.11621691997752409No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA7940.10881631422423835No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA7400.10141570847095262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA4950.0106.9228742
AGCACGC4950.0102.5639341
CACGCAC5650.093.669383
ACGAGCG259.3869935E-486.997049
CGCACTT6250.080.037285
GTAACAA19400.076.6082154
ACGCACT7250.074.997454
TAGTAAC20850.071.633172
TAACAAG21600.068.8055345
AAACACG6550.067.516795
GAAGCGG1850.062.726361
AGTAACA23850.062.6184123
GTAGTAA24750.062.417481
GAAACAC7300.061.5732464
AACACGA6500.053.5366446
ACAACGC2500.052.1982278
ACACGAG6900.050.4330647
CGAGCCT1301.3096724E-1050.19065
CAACGCA3250.049.0752569
ACAAGAG30750.048.567457
Produced by FastQC (version 0.11.5)