FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_5_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_5_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793392
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA14110.1778439913687055No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT12100.15250973037288No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC12000.1512493193780628No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA11650.14683788089620262No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG11380.1434347712101962No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG11110.1400316615241898No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC11040.13914937382781778No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT10620.13385564764958557No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT10140.12780567487446307No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG9660.12175570209934056No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA9210.11608385262266319No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT9110.114823441627846No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT8980.11318490733458367No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG8880.11192449633976648No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA8710.10978179764857725No Hit
AATGATAACTGATGGCTCAGCTTCAGGTGTTAGTGAATGCAGATTCCTTA8210.1034797426744913No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC8100.1020932905801924No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC8050.1014630850827838No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGG203.8658065E-4108.7496644
GAAGCGG2200.085.7193761
AACACGT1350.075.184966
GTAACAA10650.074.88244
TAGTAAC10850.074.838482
AAACACG4250.073.352725
GGCTAGA300.001932232172.5317841
AAGCGGA2650.071.131862
ACACGAG3300.065.908897
AACACGA3300.065.908896
AGCGGAG2950.063.8981133
CACGAGC3250.062.461358
TAACAAG12700.062.224225
GAAACAC5250.059.3807754
AGTAACA15150.055.0328333
GCCGATG400.006050500654.3748328
TAGGGGC400.006050500654.3748325
GGCGAAA3200.054.374832145
GCGGAGC3350.054.1043134
ACGTGGG450.00963852648.3331879
Produced by FastQC (version 0.11.5)