FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_56_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_56_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215418
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC23760.1954883011441331No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT21240.1747546934470281No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA18250.15015410336197094No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC12460.10251617139124154No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT12410.10210479028614024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA33350.097.607364
AGCACGC8150.088.999781
TAGTAAC36950.088.2974242
TAACAAG37000.087.978545
CGCACTT8550.086.490055
GCACGCA8400.086.3119052
AAACACG15550.080.1918265
ACGCACT9500.079.367344
AGTAACA43300.075.512833
CACGCAC9900.073.963643
CGGGTAC754.8548827E-967.668526145
GTAGTAA49400.067.102051
CGAGCGA651.398439E-766.9221655
GAAGCGG4800.066.490261
GAAACAC18950.066.186434
ACAAGAG49400.065.894857
ACGAGCG902.8194336E-1064.4435654
ACGTGCA5000.062.351722
AACACGA14050.061.920866
CAAGAGC44700.061.794468
Produced by FastQC (version 0.11.5)