FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_55_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_55_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202531
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT27020.22469275220347748No Hit
CTTCAAGGGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT26610.22128327668891695No Hit
GTTTAGGCGGTCTTGACCGGGGTAGTCAAGGGCTCTTTGCCATGAAAGCC23640.19658536869319793No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC21400.17795799027218426No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG21170.17604535766645518No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG20610.17138851306120176No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA20000.1663158787590507No Hit
AGTAGAAACAACGCACTTGGCCTCGATCACGTGAAAGATCCGAATGCGAT17180.14286533985402455No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC17160.1426990239752655No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG16660.13854112700628923No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT16470.13696112615807826No Hit
GTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATGACCACA16440.13671165233993968No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA16320.13571375706738537No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA16220.13488217767359012No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA15990.13296954506786104No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT15480.12872849015950524No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG15020.12490322494804708No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG14960.12440427731176994No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA14920.12407164555425182No Hit
GGGTAGTCAAGGGCTCTTTGCCATGAAAGCCTTTCATAGCACTCCAGAAT14760.12274111852417942No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT14520.1207453279790708No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG14370.11949795888837793No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA14340.11924848507023934No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC14160.11775164216140789No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC13850.11517374604064262No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG13560.11276216579863638No Hit
TCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTTATTCTTGACTCT13260.11026742761725061No Hit
TTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGAAC13180.10960216410221442No Hit
CTTTTGTTGTGAGTCTTTATTCTTGACTCTAATTTTCTTTTTAGTCTGTT13010.1081884791327625No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAA13010.1081884791327625No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT12330.10253373925495476No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCC300.001935646472.50181145
CAACGCA7900.069.7456749
AAACACG8500.069.087095
GTAACAA11700.068.780914
ACGAGCC4050.066.233479
AACACGA6300.065.594156
TAACAAG12500.064.378935
CACGAGC6600.062.6125958
CGAGCTA350.003566914262.1418233
GAAACAC9850.059.6182984
AACAACG9400.057.073527
ACGTGCC2300.056.738199
ACAACGC9350.056.6033368
TAGTAAC14700.055.237182
AACACGT3150.055.2371756
CGGAGAT400.00603964454.4012371
ATGCCGC553.2188682E-452.728584145
AGTAACA15400.052.72643
ACACGTG3350.051.9394347
ACACGAG8400.049.1956067
Produced by FastQC (version 0.11.5)