FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_53_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_53_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587185
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA6720.11444434036973014No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA6620.1127412995904187No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG5880.10013879782351388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC10100.082.549122
GTAGTAA11450.073.499511
AGTAACA13100.063.091313
AAACACG7250.062.99975
GTAACAA13400.060.5967334
TAACAAG13750.059.0542685
CAACGCA4550.057.3623669
ACACGAG5200.055.768977
AACACGA5400.055.046046
GTCCCGA805.842139E-754.411811
AACACGT3100.053.790076
CACGAGC5550.050.945718
ACAACGC4850.050.82458
ACGAGCA2900.049.9997639
AACAACG5200.046.00947
ATTAGTA6450.043.866891
CAAGAGC17350.042.622288
GAAACAC11050.041.334654
GTAGAAA16050.040.229711
ACAAGAG20950.039.450897
Produced by FastQC (version 0.11.5)