FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_51_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_51_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3412960
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG85630.2508965824387042No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA79570.23314073414279685No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA77690.22763231915990811No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG71700.21008157142187425No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC66390.19452322910318315No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA65650.19235502320566314No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG63120.18494210304252026No Hit
CATATGACCAGAGTGGAAGACTTGTTGCTAAACTTGTTGCTACTGATGAT59180.17339787164221088No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAATTCACTC59130.1732513712437298No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT58430.1712003656649946No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT55070.16135553888706577No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA54320.1591580329098495No Hit
CTTCAAGAGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT54140.1586306314753176No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC52630.15420631944118887No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC52440.15364961792696077No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT48030.14072828278092916No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG47420.13894097791945995No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG43720.12809994843185973No Hit
ATATGTTATCATCAATCCTTTTACTTAAAAGATAAAGTTCTTCCGTGACT42920.1257559420561624No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG42700.12511134030284563No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT42170.12355843607894614No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGTCAAGAATAAA40970.12004242651540012No Hit
GTTTAGTCACTGGCAAACAGGAAAAATGGCGGACAACATGACCACAACAC40920.11989592611691903No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG39910.11693661806760115No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGGTTGACA39760.11649711687215789No Hit
ATTATGGAGCTGTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATC39670.11623341615489194No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA39440.11555951432187896No Hit
GTTTAGGCGGTCTTGACCAGGATAGTCAAGGGCCCTTTGCCATGAAAGCC38990.11424101073554921No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC38750.11353780882284No Hit
TTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGAAA38550.11295180722891565No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT37510.10990459894050912No Hit
TTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGGGAGTCCTATCCCAAT37080.1086446955135718No Hit
CAATTGGACCGATTACCCTTCAACACCAGGGAGGTGCCTTGATGACATAG37070.10861539543387559No Hit
GTTATCATCAATCCTTTTACTTAAAAGATAAAGTTCTTCCGTGACTAGTC36790.1077949932023815No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCATCTGGTTCTTCTTCTA36530.10703319113027987No Hit
GGATAGTCAAGGGCCCTTTGCCATGAAAGCCTTTCATAGCACTCCAGAAT36420.10671089025362149No Hit
GGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGGACAACATGACCA36270.10627138905817825No Hit
CCTTTATCTTTTGCCTTGCATCTTTGTTGTTCATGTCCCTTAATACTATT35570.10422038347944307No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG35170.1030483802915944No Hit
AATTGGGATAGGACTCCCACCGCAGTTTCAGCTGCTCGAATTGGCTTTGA34860.10214007782101168No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGGACAA34480.10102667479255543No Hit
CCTATTTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTA34160.1000890722422765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA100.007091088145.003542
GCGGTCC100.00709202144.997189
ACAACGC11650.065.964388
CAACGCA12500.065.538739
GACGTAA350.003566274462.147106145
GCACGCA3450.060.943522
AGCACGC3800.059.172191
ACGTGCA3350.058.4316989
GTTTCCG400.006050494554.37872145
TCGACAA400.006050494554.37872145
CGGTGGT400.00605259954.373944
GTAACAA69550.053.6833534
TAGTAAC74600.051.1205562
TAACAAG75500.049.3566555
AGCGGTG450.00964185748.332398
GAAGCGG6800.048.000211
AGTAACA80750.047.314873
AACAACG17600.044.8996937
GTAGTAA89250.044.617341
CAGCCGT657.339787E-444.6145176
Produced by FastQC (version 0.11.5)