FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_50_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_50_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079397
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA40420.37446833741431557No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC31860.2951648003468603No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC25130.23281517365714377No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT22530.20872765071609425No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA18450.17092876856244738No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA18430.1707434799244393No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT18160.1682420833113303No Hit
CCCATACACCGGCGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA17960.16638919693124957No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA17760.16453631055116885No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA17370.16092318211001141No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACCGGCGTTCCCCC16120.14934264223450686No Hit
GATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACCGGCGT15910.1473971115354221No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA14890.13794739099701037No Hit
GATATAACCCCAGCACATGGTCCCGTGAAGAAAATGGATTATGATGCAGT13740.12729329431154618No Hit
TTCCCATACACCGGCGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCA13740.12729329431154618No Hit
ATCTGGGACTCATAGCTGGAGAACTAAAAGGAACAGATCTATACTAAATA13560.12562569656947353No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC13480.12488454201744122No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT13430.12442132042242103No Hit
GTAGAAACACGAGCCTTTTTCATTTTAATCATTTGTTTGTCACATGTATC13410.12423603178441296No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC13030.12071554766225957No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA11920.11043202825281152No Hit
GATTAAGAATGGATGCACGACTAGATTATGAATCAGGAAGAATGTCAAAG11500.10654096685464198No Hit
ATTAGATTGCGTTCTGGAGGCTTTGGATAGAATGGATGAGGAACATCCAG11390.10552187934559759No Hit
GTAGTAACAAGAGCCTTTTTCATTTTAATCATTTGTTTGTCACATGTATC11330.10496601343157337No Hit
ATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCAAGTGC11200.1037616372845209No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTACCCATACAGGCAGCAATT10940.10135288499041595No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG100.007090377145.00385145
ACAACGC4050.085.924228
AAACACG6900.075.650685
CAACGCA4650.073.278129
GCACGCA955.456968E-1268.6860352
ACACGAG6000.067.665327
CGACGTG451.1937444E-464.44615145
GAAACAC8250.064.150244
AACACGA6550.061.9835056
TAGTAAC12250.058.0015372
TAACAAG12700.055.9437755
TTTAACG400.006051489654.3739244
CACGAGC7550.052.8135228
ACGTGCA1101.6752892E-952.726239
ACGAGCG858.9267087E-751.1754539
AACACGT2500.049.2990236
ACGAGCC4650.048.332389
GTAACAA15550.045.2241864
AACAACG10100.044.504077
AGTAACA16600.042.8003583
Produced by FastQC (version 0.11.5)