FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_4_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_4_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8013494
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA198510.24771965886540878No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG194940.24326467331229049No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA178950.22331083045672712No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT167750.20933440519204233No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT164420.20517891446602443No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG147470.1840270923020595No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT146410.18270432348236612No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG144410.18020853325652955No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC136440.17026280920657083No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA130370.16268808587115682No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG130330.16263817006664008No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT126790.15822062136690934No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGT126330.15764658961496697No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA125060.15606176282156073No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA124690.15560004162978097No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG124280.15508840463348447No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC123440.1540401727386331No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC121180.1512199297834378No Hit
CATATGACCAGAGTGGAAAACTTGTTGCTAAACTTGTTGCTACTGATGAT115950.14469343834287515No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA112280.14011366327846506No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC111500.1391403050903888No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAATTCACTC108960.13597065150357635No Hit
ATATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACT107550.13421111939436156No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGTCAAGAATAAA102150.12747248578460282No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT102140.12746000683347364No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTTTTCCACTCTGG96140.11997263615596393No Hit
ATTATGGAGCTGTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATC95370.11901175691901685No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA91350.11399521856508535No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT90730.113221523595076No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC89800.112060981140062No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAAT89730.11197362848215772No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA89390.11154934414376551No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT87880.10966502252325888No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG86350.10775574300049393No Hit
GTAAGAAGTACAATAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA85420.10659520054547991No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATTCTTTTAC85060.10614595830482933No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA84280.10517260011675307No Hit
GTATTGTCCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAA83310.10396214185722233No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG82860.1034005890564091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC25450.0114.293231
GCACGCA26300.0110.542062
CACGCAC27400.0106.630783
GAAACAC22200.084.58144
AAACACG22750.082.5365755
ACAACGC25250.081.255578
CAACGCA25700.080.11499
GTAACAA120800.076.819444
AGCGAAG851.8189894E-1276.764862
AACACGT6650.076.314056
ACACGTG6900.073.549047
ACACGAG20500.067.547247
AACACGA21050.065.782346
TAACAAG145700.063.7905775
GCGAAGC1051.6370905E-1162.1414383
CACGAGC21850.061.3830348
GCGTCAG606.788967E-660.44811
CGCACTT50150.059.9936455
TAGTAAC159200.059.6122472
ACGCACT50800.059.226014
Produced by FastQC (version 0.11.5)