FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_49_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_49_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729452
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG31530.18231208498414528No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC31070.1796522829196763No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT29620.17126812423819798No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA27010.15617663861153705No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG25470.14727208387396704No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA23330.13489822209578525No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA22290.12888475655872497No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC22180.12824871693461282No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG20740.11992238003714473No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG20450.11824554830084906No Hit
GGCTTGGGCTGTCCCAAAGGACAACAACAAAAATGCAACGAACCCATTAA20190.11674218191658399No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC19500.11275247881988053No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT19450.11246336989982954No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19360.11194297384373778No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC19220.11113346886759505No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA18870.10910970642723822No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT18420.10650772614677945No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT18240.10546693403459594No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT17880.1033853498102289No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA17680.102228914130025No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC17650.10205544877799441No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA17500.10118812201784148No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGGTAGCGTTAGGGCA17330.10020515168966818No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17320.10014732990565797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGGC100.0070784334145.088151
GCGGTGC203.8572025E-4108.816111
GCACGCA2950.0105.678012
AGCACGC3150.099.028421
CACGCAC3250.095.920343
ACACGTG2050.095.485087
AAACACG8300.093.46125
GAAACAC8600.090.200924
AACACGT2200.088.974736
GCGGGCA259.387901E-487.000032
CCCGGCA259.3889795E-486.997524
ACGGACC950.083.95225145
CAACGCA3300.083.482479
ACAACGC3400.081.02718
ACACGAG7400.079.355857
AACACGA7400.079.355856
GTAACAA32350.077.76444
CCGGCAA300.001936201172.497935
TAACAAG36850.068.6615455
ACGAGCC3400.068.233359
Produced by FastQC (version 0.11.5)