FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_48_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_48_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129022
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT56350.4991045347212012No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC42990.3807720310144532No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT36920.3270086853931987No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG25730.22789635631546593No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA24820.21983628308394343No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA23260.2060190146870477No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC22160.19627606902257No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT21300.18865885695761464No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC13580.12028109283964351No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT13040.11549819224071808No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC13010.11523247554077777No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG12830.11363817534113596No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG12510.11080386387510607No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT12450.11027243047522547No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG12400.1098295693086583No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC12380.10965242484203143No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA12330.10920956367546425No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT11670.10336379627677761No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA11330.10035234034412086No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT100.0070903082145.00461145
AGCACGC9300.0120.094821
GCACGCA9500.0116.766872
CACGCAC9800.0113.1873553
GAAACAC9650.0112.692894
AAACACG10500.0103.570145
GTAACAA27950.097.530094
CGCACTT12050.096.264365
ACGCACT12250.095.2845154
TAACAAG33050.082.26075
AACACGA11500.077.542516
AACACGT2550.076.763756
TAGTAAC37000.075.049682
AGTAACA39250.071.298463
ACACGAG12550.071.0548867
GAAGCGG3450.069.371531
CACGAGC13400.066.5476768
TAGAAAC19500.065.8097842
GTAGTAA45350.062.369721
ACACGTG3350.058.4321067
Produced by FastQC (version 0.11.5)