FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_46_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_46_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204903
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA22070.18316827163680396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG1050.089.761295
CGTCTAA259.3874754E-486.99948
AAGCGTC259.3874754E-486.99945
TAGTAAC20250.077.697272
AGCGTGC1350.069.814348
AACACGT4800.069.478686
GTAGTAA23850.067.221671
CAACGCA6100.064.1798869
AAACACG13950.063.924295
GCGTCTA350.00356677862.142437
AGTAACA26500.059.3675163
GCGTGCT1600.058.905859
ACGAGCA3850.058.3762259
GAAACAC15550.057.8131074
ACAACGC5800.057.4996038
TAACAAG28800.054.626365
TAAGCGT400.00605134954.374634
AACACGA10400.054.3746266
CACGAGC10400.053.6775178
ACGAGCC4750.051.8943829
Produced by FastQC (version 0.11.5)