FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_42_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_42_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079572
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT24980.23138799450152467No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC23280.21564101329045213No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT22590.2092495915047815No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT22340.20693385897374142No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC20480.18970480894280325No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC15020.13912921046488794No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT14320.13264515937797572No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC14170.13125571985935167No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT14140.13097783195562684No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC13820.12801369431589554No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC13640.1263463668935467No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC13230.12254856554264097No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA13060.12097386742153371No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC13060.12097386742153371No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT12970.12014020371035929No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT12910.11958442790290967No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA12870.11921391069794325No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG12640.11708343676938637No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT12450.11532348004579591No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA12280.11374878192468867No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG12090.11198882520109821No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT11830.10958046336881652No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA11780.1091173168626085No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA11670.10809839454895087No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA11400.10559740341542759No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG11390.10550477411418599No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT11320.10485636900549476No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG11270.10439322249928674No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA11040.10226274857072987No Hit
CCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT10820.1002249039434146No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG10800.10003964534093139No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC1650.0127.466351
GAGCAGA1800.0120.873251
CACGCAC1750.0120.1380163
GTAACAA19150.0118.115354
GCACGCA1800.0116.806272
GAAACAC6450.0115.770534
AAACACG6900.0109.2709665
TAACAAG24000.094.24625
ACGAGGA259.388453E-486.99654
AACACGA6850.085.726486
AACACGT1950.085.5093846
TAGAAAC9900.080.5560462
TAGTAAC28900.079.775572
ACACGTG2150.077.5550167
GGAGCGT851.8189894E-1276.761626
CGGAGCG851.8189894E-1276.761625
CGCACTT2850.073.768965
CGTGCCT502.306917E-672.497083
AGTAACA32100.072.045393
CACGAGC8300.070.750168
Produced by FastQC (version 0.11.5)