FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_40_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_40_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936702
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC29950.31973882835736445No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT29390.3137604061910832No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT25370.27084387564027834No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC24380.2602748793106025No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT23060.2461828842043681No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA17280.18447702684525066No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC16300.17401478805425843No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC15730.1679296083492936No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT14820.1582146723290865No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC14790.15789439971303573No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG14670.1566133092488326No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG13740.14668485815125834No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA13330.142307799065231No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT13300.1419875264491802No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC13030.13910507290472315No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA12800.1366496495150005No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC12760.13622261936026614No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC12600.13451449874132862No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA12510.13355368089317626No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT12320.131525287658188No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA11880.12682795595610985No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG11690.12479956272112155No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG10740.11465759654618012No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA10530.11241568823382464No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC10330.11028053746015275No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT10280.10974674976673479No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT10220.10910620453463322No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA10170.10857241684121524No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC10010.10686429622227775No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT9710.10366157006176992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA202.1291708E-6145.018692
CGTCAGA100.007088059145.018692
GTTAGGT100.0070914538144.995479
GACGCAC100.0070914538144.995474
GTAACAA21800.0111.074514
AAACACG5200.0105.958225
AGCACGC1400.0103.6567151
GAAACAC5550.099.276184
TAACAAG25400.095.3316655
GCACGCA1600.090.636682
CACGCAC1700.085.291453
GAAGCGG2150.084.371751
AACACGT1650.083.482246
TAGTAAC30500.079.879142
CGGAGCA4050.075.194882
AGTAACA33650.072.605463
ACACGTG1900.072.4977347
ACGAGGA406.6639994E-572.4977344
ACAAGAG33700.071.852357
AACACGA5800.071.247776
Produced by FastQC (version 0.11.5)