FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_39_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_39_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755168
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTAAAAACAATTTTAGGTAGAAAACAGAGTGTGAGTT13300.17611975083689987No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG10900.14433874316708337No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC8480.11229289376668503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC11650.079.66162
GTAGTAA12200.076.700141
AGTAACA14050.066.0495453
AAACACG11000.061.9542925
AACACGT4000.061.624756
AACACGA7350.059.183436
ACGAGCA3400.053.3086059
ACACGAG8250.052.7270557
CACGAGC8400.051.7855078
ACACGTG5100.049.75477
CGGAGCG752.550047E-548.3331345
GTAACAA19950.046.5160984
ACAACGC3700.045.067388
ACGAGCC3450.044.1302579
TAACAAG21500.042.8254055
TAGAAAC22000.042.184442
ACGTGCA2850.038.1602672
CAAGAGC23600.037.7858628
AACAACG4650.037.41927
TAGGCCG800.002036340836.249855
Produced by FastQC (version 0.11.5)