FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_38_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_38_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353967
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT32380.23914910776998258No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC30340.22408227083821097No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT25250.18648903555256516No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT24750.18279618336340545No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21590.1594573575279161No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA20200.14919122844205213No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC17110.12636940191304513No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT16960.12526154625629723No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC16910.12489226103738127No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC16570.12238112154875266No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA16300.12038698136660642No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT16260.12009155319147366No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA15770.11647255804609713No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA15660.11566013056448199No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT15580.11506927421421644No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA15550.11484770308286686No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT15090.11145027906883993No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG15050.11115485089370715No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG14450.1067234282667155No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT14420.10650185713536593No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG14390.10628028600401634No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC14260.10532014443483481No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT14160.1045815739970029No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC14120.1042861458218701No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC14030.10362143242782136No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA100.0070907273145.0028145
GTAACAA20200.0116.643994
AGCACGC3700.0109.821081
GCACGCA3800.0106.844182
CACGCAC4100.099.0226443
AAACACG7100.095.984235
GAAACAC7000.095.284044
TAACAAG27550.085.524815
CAACGCA2850.078.858269
CGCACTT5600.075.087975
TAGTAAC32350.074.1822742
GAAGCGG2650.073.929421
AACACGT1900.072.4987266
ACGCACT5850.071.8790744
TAGAAAC11600.070.001362
ACGTGCA2600.069.712892
AGTAACA37150.064.7902453
AACACGA8300.064.637416
GTCTCGC350.003555036862.1945881
GTAGAAA13650.059.53671
Produced by FastQC (version 0.11.5)