FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_37_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_37_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614652
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCGGGGGTTGGAGGAGTTGAGGGTGAAAGTCCGGGCAA8970.14593623709025594No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCG259.3861023E-486.997469
GTAGTAA8200.073.417841
TAGTAAC8650.069.5644452
CGCACTT3100.067.82065
AAACACG8650.066.2119455
TAACAAG10350.057.4379355
CAACGCA2000.054.3734139
ACAACGC1750.053.855578
AACACGT3850.052.7257356
ACACGAG7200.052.3595857
GTAGATA1750.049.7128334
ACGCACT4600.048.857274
GTAACAA12550.047.9468084
AACACGA7750.047.7082826
CACGAGC7700.047.076558
GCACGCA4950.046.867322
AGTAACA13250.045.9609223
TAGATAG2100.044.8796465
ACGAGCA3950.044.0493479
ACGAGCC2700.042.9617089
Produced by FastQC (version 0.11.5)