FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_35_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_35_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948673
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA12250.076.390891
TAGTAAC12900.069.693292
AAACACG12150.062.0573435
AACACGA7500.059.9330756
ACACGAG7800.057.6279567
CGGTCGC400.00605089554.374769
ACGAGCC4900.051.7854929
GTAACAA18150.049.9309124
TAACAAG18350.048.9916085
GAAGCGG4150.048.9438251
AACACGT6500.046.845956
GTAGAAA24100.045.752471
ACACGTG6900.045.180967
GCACGCA2250.045.113292
CAACGCA3800.043.8813869
AGTAACA20800.043.5695233
TAGAAAC24100.043.3215982
ACAACGC3850.043.31158
CGGAGCG855.3414235E-542.6468735
CACGAGC10250.042.438848
Produced by FastQC (version 0.11.5)