FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_32_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_32_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015002
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT31160.3069944689764158No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT24850.24482710378895806No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA23470.23123107146586905No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA20420.20118186959237522No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC20160.19862029828512656No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA20150.1985217763117708No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA19920.19625577092458932No Hit
GCTCTGAGATCATCCACTTTCTCATCTAGTTCTAGTATTTCGTTGTGGAG18200.1793099915074059No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA17970.1770439861202244No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC17910.17645285428009008No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC17740.1747779807330429No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA17730.17467945875968716No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA17150.16896518430505555No Hit
CCCATACACAATTGAATCTATCATTATTAACTTTCAAATTTTTGAGCCTG16470.16226569011686676No Hit
GTTATAATTTTACCTATTTCCCAACTTTTTGTTATTGGATCTCTCCACCA16310.16068933854317527No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA16040.15802924526257092No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA14910.14689626227337482No Hit
GAGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCATAAACAAG14830.1461080864865291No Hit
GCCCAAGCCATTGTTGCGAAGAATCCGTTTCCATTGGTAACGTTAGGGCA14630.14413764701941473No Hit
CTTCTATTGTTAATGGATTTGTTGCTGTTTTGTTTTTGTCGTTTTTTGGG14310.14098494387203178No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC14230.14019676808518605No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG13840.13635441112431307No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC13280.13083718061639288No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA13110.12916230706934567No Hit
GCTATCATCAGTGTTACAGCCAAACTGGAGGCAGCAGTTGAGTAGTAAAG13050.12857117522921138No Hit
TTCTCATCTAGTTCTAGTATTTCGTTGTGGAGTTCATCCATGGCACCGCT12990.12798004338907706No Hit
ATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATATTCAGTGAATCAA12970.12778299944236562No Hit
CTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTA12890.1269948236555199No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA12820.12630516984202986No Hit
GTTTATGGCCTCTTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCAT11580.1140884451459209No Hit
GATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGG11520.1134973133057866No Hit
ATATAGACCTCCTGCAAAACTATTAAAGGAAAGGGGTTTCTTCGGAGCTA11450.11280765949229657No Hit
AATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAAGGTACCAGCAGCT11380.11211800567880655No Hit
GGCCAGATGTTACCAGCAGCTGAACAGCATCTACAGAAACTAGCTGCAAA10950.1078815608245107No Hit
CTCAAAGAGTTGAGATTTTTTGTTATCTTGTTTATGGCCTCTTGAGTGCT10950.1078815608245107No Hit
GTTCTAGTATTTCGTTGTGGAGTTCATCCATGGCACCGCTTAGTCTTTGA10900.1073889509577321No Hit
GTAATAAAAGGATCCTTGCCTTTAATTGGAGAAGCAGATTGCCTCCACGA10860.10699486306430922No Hit
TACTAGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGATACAATAAGC10620.104630335703772No Hit
CCATATAAACAACAAAGATAGCTATCATCAGTGTTACAGCCAAACTGGAG10560.10403920386363771No Hit
AATATAGACCTCCTGCAAAACTATTAAAGGAAAGGGGTTTCTTCGGAGCT10550.103940681890282No Hit
TATTGTATCAGCTCTGAGATCATCCACTTTCTCATCTAGTTCTAGTATTT10540.10384215991692627No Hit
GCCTAACCTTCTCCGAGGATACGAACATATCAGGTTATCAACTCACAACG10460.10305398413008053No Hit
CTCTATGCCAAGTTATAATTTTACCTATTTCCCAACTTTTTGTTATTGGA10410.10256137426330195No Hit
GGATTGGAAGGAATGATTGCAGGTTGGCACGGATACACATCCCATGGGGC10380.10226580834323479No Hit
GGATTACGGGTAGCCCATACACAATTGAATCTATCATTATTAACTTTCAA10260.10108354466296617No Hit
TTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGCTT10240.10088650071625475No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG10230.10078797874289902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTC900.096.664179
AACACGT3650.085.408766
ATTAGTA3950.084.498961
CAACGCA1700.081.027329
GTAACAA14650.077.199374
CCGCGAG300.00193594972.498138
TAGGGTA1050.069.045845
TAACAAG17000.067.3806155
ACACGTG4850.065.77157
ACACGAG3650.061.5737577
TAGTAGT3100.060.8048823
AAACACG9250.057.214745
CACGAGC3950.053.2264758
CTAGGGT1500.053.1652954
ACAACGC2650.051.979798
TAGTAAC23200.050.6261832
GTAGAAA13900.050.1126171
AACACGA4450.048.8751456
CCGTAAC450.00963828448.334472
ACGTGCC1351.9099389E-1048.3320859
Produced by FastQC (version 0.11.5)