FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_31_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_31_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773788
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT16760.21659679395389952No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC15180.19617776445227894No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA11350.14668100306543913No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT10710.13841000377364343No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT9550.12341881755726374No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC9210.11902484918349727No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGCATCGCATGTAT8330.10765222515727822No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCA100.007091735144.991935
AGCACGC4000.0114.247581
GTAACAA19050.0108.0779654
GCACGCA4700.097.175442
CGCACTT5050.094.747195
TAGTAAC22450.092.678572
TAACAAG22500.092.150425
ACGCACT5750.083.212754
CACGCAC5600.081.557953
AGTAACA26200.079.413513
AAACACG9550.078.948485
GAAACAC10200.073.917454
ACAAGAG30400.067.9649667
GTAGTAA32100.066.662791
CAAGAGC27950.064.844328
ACGAGCG451.1940175E-464.440859
TAGAAAC14350.063.149792
CGAGCTA702.3347457E-762.1393975
ACGTGCA3350.060.5936362
AACACGT3900.059.4838646
Produced by FastQC (version 0.11.5)