FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_28_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_28_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656096
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT22540.3435472857630591No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC18900.2880675998634346No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT17190.2620043408281715No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT14270.2174986587328684No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT14040.21399307418426572No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT11540.17588889430815002No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC11330.17268814319855633No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT9610.14647246744378872No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA8520.12985904501780227No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC8350.1272679607862264No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC8280.12620104374969518No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT8170.12452445983514608No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT8080.12315270935960591No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC8050.12269545920109252No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC7810.11903745793298542No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG7790.1187326244939765No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT7670.11690362385992294No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT7660.11675120714041848No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA7610.11598912354289616No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC7550.11507462322586938No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA7440.1133980393113203No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT7390.11263595571379797No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA7380.11248353899429352No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC7360.11217870555528459No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT7330.1117214553967712No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA7210.10989245476271765No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG7200.10974003804321318No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG7140.10882553772618642No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA7080.10791103740915964No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA6940.10577720333609715No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA6790.10349095254353022No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG6650.10135711847046773No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCAT100.0070758783145.098851
GAGCAGA950.0129.825291
GTAACAA12950.0123.725294
GCACGCA2000.0123.249412
GAAACAC4000.0123.249414
AGCACGC2050.0120.325871
CACGCAC2100.0117.3803943
CAGCGTT256.463355E-6115.999455
ACAGCGT256.463355E-6115.999454
AAACACG4500.0109.555035
TTTACAG501.9463187E-10101.499523
TAACAAG16500.097.10565
CACAGCG301.5990849E-596.666213
GAAGCGG1450.090.061351
ACGCACT2750.089.635944
CAGCCTT651.6370905E-1189.2303545
CGCACTT2800.088.03535
TAGTAAC18600.086.142072
TAGAAAC6100.084.384852
CAGCATC353.4364748E-582.856754
Produced by FastQC (version 0.11.5)