FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_26_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_26_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115127
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC20570.18446329431535602No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT20080.18006917597726538No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG19700.17666149236813386No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA16560.14850326465057345No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT16080.14419882219693364No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT15960.1431227115835237No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG14990.13442415079179323No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA14880.1334377160628341No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC14180.12716040415127605No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG13860.1242907758488495No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT13850.12420109996439867No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA13020.11675800155497983No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC12960.11621994624827485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA4250.097.234692
AGCACGC4400.093.957791
GTAACAA30150.078.631354
AAACACG7600.078.22325
AGCGTGC1500.077.3328558
TAGTAAC31500.075.7217562
CACGCAC5500.075.1358953
TAACAAG32050.073.9698945
CGCACTT5900.071.2707445
GTAGTAA35450.068.539221
ACGCACT6750.066.592184
CGGAGCG1200.066.4579245
AGTAACA36250.065.799593
ACAACGC2550.065.3917548
AACACGA7200.064.4440466
GAGCGTG2100.062.142477
GAAACAC10300.059.1258434
GAAGCGG4800.058.929671
TGCACGG502.0104066E-457.999645
GCGTGCT2000.054.3746649
Produced by FastQC (version 0.11.5)