FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_24_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_24_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865305
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA12670.14642235974598553No Hit
GTAGTAACAAGAGCACAAGGGGCAAAGATGACGACAGTTTTGGCTACTTC11470.13255441722860725No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA11290.13047422585100052No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC9390.10851665019848493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA16350.085.195171
TAGTAAC16300.084.512572
CGCATAG300.001935348172.502884145
ACGTGCG1000.072.4986959
AGTAACA21600.063.7720073
AAACACG10500.063.5226675
GTAACAA21950.063.0854264
AGCGACG753.7610698E-757.9989628
AAGAGCG4150.057.649579
ACACGTG4450.055.392267
TAACGTA1051.1095835E-955.2371067
TAACAAG25200.054.6617165
ACACGAG7950.052.8921367
AACGTAC1101.6752892E-952.7263268
AACACGA8050.052.2350856
CACGAGC8150.051.594178
ACGAGCC3550.051.0554249
ACAACGC7400.050.945038
ATAACGT1152.4847395E-950.433886
ACGTACC1152.4847395E-950.433889
Produced by FastQC (version 0.11.5)