FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_23_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_23_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985122
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA16940.17195839703102764No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA12960.1315573096530176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA19450.088.75941
TAGTAAC21050.081.9712142
AAACACG10050.080.0743565
ACGAGCG702.8121576E-972.499749
ACACGAG7550.069.1388247
AGTAACA25500.067.09783
CGGAGCG554.0616433E-665.908865
AACACGA8200.065.42666
GTAACAA27150.062.2189374
TAACAAG28800.058.654315
ACAACGC5350.058.2708248
AACACGT5100.056.8625456
CACGAGC8500.056.2939228
CAACGCA6000.054.374819
AACAACG5800.053.749817
GAAGCGG7400.049.0111961
ACGAGCC2600.047.403689
GTAGAAA26350.047.0730671
CAAGAGC33450.046.8159838
ACAAGAG36850.046.2345777
Produced by FastQC (version 0.11.5)