FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_22_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_22_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633889
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA34160.2090717300869276No Hit
GTACTGACCAGCCATCTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG32350.19799386616838718No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA28670.17547091632295705No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA28130.17216591824781244No Hit
AGTAAGATGTTTGCCTTTTCCCCTGGTTTCAGCTTTTCAAGTTCTTCAAT25110.15368241049422574No Hit
GTATTAGCAGGCTTTCTCGTTAGTGGCAAATATGACCCAGATCTTGGAGA23390.14315537958820948No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA22330.13666779077403668No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT20650.12638557454025334No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT19950.12210131777617697No Hit
AAGCAGAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA19860.12155048476365285No Hit
GTTCCAATGGGATGCATTTGAAGCATTTGAGAGCATAATTCCTCAGAAGA19270.11793946834821704No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA19000.11628696931064472No Hit
CCAAAGAACTGGTGCAAAACACTTATCAATGGGTGCTAAAAAACTTGGTG18560.11359400791608242No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT18370.11243113822297598No Hit
GTCCTAACTATATGCGGCAGAATGATGTCATTAAAAGGGAAAATTGAAGA18350.1123087308868595No Hit
GGTTTCAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG18320.11212511988268482No Hit
ATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGC17960.10992178783258838No Hit
CATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTG17960.10992178783258838No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG17170.10508669805598789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC20900.084.6394352
AAACACG17750.078.829395
GTAACAA23550.073.883854
GTAGTAA26150.071.290221
AACACGA13350.067.882526
AGTAACA26100.067.7764053
AACACGT7500.067.66536
ACACGAG13500.067.665297
GAAACAC21850.065.364814
TAGAAAC28500.064.86712
TAACAAG28950.061.3545385
CACGAGC14600.060.5809638
ACAACGC6500.059.1141858
ACGAGCC4350.058.3321539
ACGAGCG1154.0017767E-1156.737989
ACGAGCT4600.055.1619269
GTAGAAA34650.054.639381
AACAACG7200.053.3669747
CAACGCA7400.050.9449129
AAGAGCG4500.049.9434289
Produced by FastQC (version 0.11.5)