FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_1_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_1_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753197
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC24100.31996941039329685No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21080.2798736585514812No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT20170.2677918260428546No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA19430.2579670391677078No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT16940.22490795900674063No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT16890.22424412205571714No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG14660.19463699404007184No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13860.18401560282369683No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC13720.18215685936083123No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC13550.17989981372735156No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT12980.17233207248568436No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG12400.1646315638538125No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT11550.15334633568641406No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA11500.1526824987353906No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA11180.1484339422488406No Hit
AAAATGAACAATGCTACCTTCAACTATACAAACGTTAACCCTATTTCTCA10820.14365431620147187No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT10290.13661764452062342No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT10270.13635210974021406No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC9710.12891713588875153No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA9280.12320813810994999No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC9180.12188046420790312No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT9150.12148216203728905No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA8930.11856127945278593No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC8780.1165697685997156No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT8780.1165697685997156No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC8770.11643700120951092No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC8720.11577316425848748No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA8710.1156403968682828No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC8700.1155076294780781No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT8610.11431272296623593No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG8600.11417995557603124No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT8470.11245397950337031No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA8240.1094003295286625No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG8160.10833819040702498No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT8130.10793988823641092No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG8100.10754158606579686No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT7960.10568284260293125No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA7940.10541730782252187No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA7840.10408963392047499No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG7810.10369133174986092No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA7630.10130151872617654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC550.0118.694621
GTAACAA18300.0108.547554
AAACACG5000.098.596025
GAAACAC5000.097.146094
TAACAAG20500.096.898545
AACACGA4650.084.1901556
GCACGCA801.8189894E-1281.559212
ACACGAG4950.079.0877157
CGGAGCA4200.075.949322
TAGTAAC26700.075.755372
AGTAACA28600.070.722683
GTAGAAA7850.069.301541
CACGCAC1009.094947E-1265.247373
ACAAGAG30300.065.079897
CACGAGC5800.064.997388
AACACGT1600.063.4349446
TCTACCC350.003566731562.140353
GCGGAGC5500.060.666141
ACGAGCC851.3051249E-859.703489
AGCGTTT2000.057.997668
Produced by FastQC (version 0.11.5)