FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_15_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_15_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021648
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT61610.6030452758680093No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT57980.5675144472460182No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT51370.5028150595899957No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT47350.4634668692152287No Hit
GAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAAC43430.42509748954630167No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT38170.37361204641911894No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA36030.35266549731414343No Hit
AAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACA35830.35070787590246344No Hit
CACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA35670.3491417787731195No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC34730.3399409581382237No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT30130.2949156656695848No Hit
ATGTTATATTGGTCTACTGTTGTTTGCTTCAAAACTGTTTTGGCCTCATT27870.27279454371760137No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT27420.2683898955413215No Hit
CTCTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT27200.26623651198847353No Hit
CATCTTGCTTTATCAAGGAAAACCAGTTAAAGTAGTGAAAAGGAAAAGGT21320.20868244248508291No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT21040.205941772508731No Hit
AGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAA20900.20457143752055504No Hit
GGATGAAGTGGGCCATGTGTTCTAACTTTCCCTTGGCTCTACCCAGATCT20020.1959579033091632No Hit
GTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT18830.18431005590966754No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT18380.17990540773338762No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC17940.17559864062769173No Hit
GAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAAC17120.16757239283980394No Hit
ACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGCTGAAA16940.16581053356929196No Hit
AACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATT16940.16581053356929196No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT16550.16199317181651607No Hit
GTATAGTGCTTTGTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAA16360.1601334314754201No Hit
AATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTG16270.15925250184016412No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT16210.15866521541666015No Hit
GTTAAAACAACTGCTAAGGGACAATGAGGCCAAAACAGTTTTGAAGCAAA16180.15837157220490813No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT15740.15406480509921225No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG15530.15200930261694828No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT15530.15200930261694828No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG15510.1518135404757803No Hit
GCTAAGGGACAATGAGGCCAAAACAGTTTTGAAGCAAACAACAGTAGACC15440.15112837298169232No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT15050.14731101122891643No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT14980.14662584373482843No Hit
GCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTC14450.14143814699387655No Hit
TCATTGGACAAAGCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTT14410.14104662271154056No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA14340.14036145521745258No Hit
ATTCAATACATCAAGGATTGAAAAGAATCCTTCACTAAGGATGAAGTGGG14240.1393826445116126No Hit
GTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT14100.13801230952343665No Hit
GAATTAAGAGACAAAGAATGACAGTTGAGTCTATGGGGTGGGCCTTGAGC13270.12988818066496483No Hit
ACTTTAACTGGTTTTCCTTGATAAAGCAAGATGTTTGCCTTTTCCCCTGG12890.1261686999827729No Hit
TCTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATACCTTTTCCTT12560.12293862465350101No Hit
GCTCTACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGC12520.12254710037116502No Hit
GACCAATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAA12430.12166617073590903No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA12410.12147040859474105No Hit
CTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATACCTTTTCCTTT12210.1195127871830611No Hit
AGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAA11290.11050772868933331No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA11140.10903951263057335No Hit
GTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAATGACAGTTGAGT11010.10776705871298137No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC10990.10757129657181339No Hit
GTTCTAACTTTCCCTTGGCTCTACCCAGATCTTGGAGATTTCAAAACTAT10980.1074734155012294No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT10970.10737553443064538No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA10780.10551579408954942No Hit
GGTTTCAGCTTTTCAAGTTCTTCAATAGTTTTGAAATCTCCAAGATCTGG10760.10532003194838144No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC10670.10443910231312548No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC10560.10336241053670149No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT10420.10199207554852552No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA10390.10169843233677352No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT10360.10140478912502153No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA10230.10013233520742956No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCCC100.0070902556145.0043145
GGTGCGT151.2301072E-4144.997223
AGCACGC6950.0126.32571
GTAACAA26150.0124.204124
GCACGCA7150.0122.695952
CACGCAC7300.0120.168923
TAACAAG27750.0117.042795
GCAGCGT203.8535957E-4108.8384551
CGGTGCG203.865625E-4108.753232
AGCGTCA203.8663775E-4108.74793
GCGTCAG203.8663775E-4108.74794
CGTCAGA203.8663775E-4108.74795
GAAACAC12600.0108.172524
AAACACG13050.0104.442445
TAGTAAC33800.096.955542
ACCGAAA301.5992397E-596.66954145
GAGCAGA2350.095.7160951
GTAGAAA15750.093.059761
CGCACTT9700.091.931225
ACGCACT9750.091.4597854
Produced by FastQC (version 0.11.5)