FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_13_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_13_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840433
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA30880.36742964638466125No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG27500.3272122822402262No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA26190.3116250789771463No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC16860.2006108755843714No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT16820.20013493044656744No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG14590.17360098901399637No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG14480.17229213988503544No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA12300.14635312987471932No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA11010.13100389918054145No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT10880.12945707748267857No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC10810.12862417349152164No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT10290.12243688670007008No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG10290.12243688670007008No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC10040.11946222958879531No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG9660.11494075077965764No Hit
CCTTTATACAAAGGAGCAGATATGCAGAATGAATAAGTCTAGAAGATCTA9420.11208507995283383No Hit
GTAGTAACAAGAGCATAGAAAACCAAGCAAATGAGAAAAACAATGTAATT9030.10744461485924517No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG9000.1070876560058922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA25900.074.2227251
ACGAGCG406.663001E-572.4990549
TAGTAAC27750.068.71492
ACAACGC7850.062.801738
TATACCG350.003566490762.1420445
AACAACG8050.061.2414327
AAGAGCG5350.058.2702649
ACGGACC1007.185008E-1058.00269145
AGTAACA33350.056.9557723
GTAACAA34450.054.5058174
ACCGCCC400.006050864754.3742878
TAACAAG36100.052.2153785
CAAGAGC36200.049.267318
CAACGCA11200.049.195789
ACAAGAG40500.046.54267
AACAAGA44550.042.311456
CACGAGC9300.040.5371068
ACGTGCC3450.039.9270139
AACACGA9450.039.8936546
ACACGAG9550.039.4759257
Produced by FastQC (version 0.11.5)