FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_12_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_12_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246548
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA42940.3444712919197656No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA17680.14183168237404414No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA17110.13725905460519772No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG16950.13597550996832852No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG16790.13469196533145936No Hit
GATAATGGCCTCTGTCAAAGCAAAGAATAGTTGTAGAGACACAAACTGGG16740.13429085763243775No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT16230.13019955910241723No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA15480.12418294361709298No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG13360.10717597717857635No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT12840.10300445710875153No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG12830.10292423556894721No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT12630.10131980477286073No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC12510.10035714629520885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA28150.091.2247541
AACACGA9400.090.236976
TAGTAAC28400.089.6014942
AAACACG16100.086.0070345
ACACGAG9700.085.9513247
ACGAGCA5100.079.605739
GTAACAA32450.078.195154
GAAACAC18750.074.624694
CACGAGC11100.074.457488
TAACAAG34350.074.0815
AGTAACA34950.073.0166553
AACACGT7450.072.0115056
AGCACGC2500.069.640051
TAAGTCG451.1941305E-464.442735
CGCACTA451.1941305E-464.442735
TAGAAAC30900.058.6554072
ACAACGC5300.057.45138
ACGAGCC4450.057.0209589
AACAACG5350.055.5592737
GCACGCA3150.055.2366262
Produced by FastQC (version 0.11.5)