FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_121_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_121_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6413516
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT296750.46269472158485303No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC237190.3698283437665081No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT230800.3598650100818334No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG125430.19557135274941234No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA118040.18404881191533629No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA101070.15758906658999525No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT98530.1536286804305158No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC94880.14793757433520086No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC67980.10599490201630432No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA67230.10482549665425331No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC66980.10443569486690296No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT66960.10440451072391493No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG64670.10083392635178581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC60050.0118.258471
GCACGCA60950.0116.8100362
CACGCAC63400.0112.179123
GTAACAA171400.0101.769364
AAACACG59400.096.7874455
CGCACTT75700.096.53775
ACGCACT79150.092.3297964
GAAACAC62700.091.577734
TAACAAG204150.085.585455
TAGTAAC212950.082.663442
AGTAACA232000.075.998993
GTAGTAA246000.072.9208151
GAAGCGG17650.072.742981
AACACGT18450.067.587186
ACAACGC17700.066.7646568
ACGTGCA24400.065.963782
CTCGCTC16300.064.937793
GTCTCGC17800.062.3496481
AACACGA73100.062.0853586
CGGAGCG3500.060.0706375
Produced by FastQC (version 0.11.5)