FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_11_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_11_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686734
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT52200.7601196387538698No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC45230.6586247367976538No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA30630.44602422480902354No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC21850.3181726840377788No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA21120.3075426584383473No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT20770.3024460708221815No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT19250.2803123188891186No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG15750.22934644272746071No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA13730.1999318513427324No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG13620.198330066663366No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT13000.1893018257433009No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA12450.18129290234646894No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT11900.17328397894963699No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT11830.17226466142640381No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG10940.15930476720243938No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA10860.15813983289017292No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG10590.15420817958627359No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA10060.14649048976750825No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA9540.1389184167377762No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT9530.1387727999487429No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC9520.13862718315970957No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG9330.1358604641680767No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT9130.13294812838741057No Hit
GTTTATATGGTCTCCAGAGACAACGTTTCATGCTCCATCTGTCTATAAGG9070.1320744276532107No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA8950.130327026184811No Hit
GAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAAC8600.1252304385686452No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT8550.12450235462347867No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT8540.12435673783444536No Hit
ATTGTATTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACC8140.11853206627311302No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT7450.10848450782981475No Hit
CACGTGCATTTTTCACTCAATTGTATTCATTGAATTAATGGATAAATTTA7310.10644587278334844No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA7260.10571778883818189No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG7190.10469847131494874No Hit
CACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGATAAATTTA6950.10120366837814933No Hit
AACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACA6940.10105805158911602No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA6940.10105805158911602No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTGAT6920.10076681801104939No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGCG100.007090851144.99683
ATACCGG100.007090851144.99683
TACCGGA100.007090851144.99684
AGCACGC9950.0126.8457641
GCACGCA10200.0123.682752
CACGCAC10350.0122.581843
GTCTCGC256.4477645E-6116.056591
GAAACAC7050.0115.174774
AAACACG7300.0111.230425
GTAACAA19950.0110.837144
AACACGT2300.097.7152256
CGCACTT13400.095.762815
ACGCACT13650.094.540044
TAGTAAC24700.089.822382
CTCGCTC259.38631E-486.9980853
TAACAAG25650.086.206675
AGTAACA25850.085.5396963
ACACGTG2650.084.809457
GTAGTAA27000.083.281341
CAAGAGG9250.077.592888
Produced by FastQC (version 0.11.5)