FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_117_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_117_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151641
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT71290.22619962108628489No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC63100.2002131587956877No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC45460.14424231693901685No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC43770.13888003106952854No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT42510.13488211379405207No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA42060.1334542861956676No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT41740.1324389421257053No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT40850.1296150164311227No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA39860.1264737957146769No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG39290.12466521409005657No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC38680.12272971445669097No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC37860.12012789527741262No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA37680.11955676423805885No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG37590.11927119871838195No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA35860.11378199484014836No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA35530.11273492126799975No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT35410.11235416724176389No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG34750.11026002009746669No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT34730.11019656109309404No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT34490.10943505304062233No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT34430.1092446760275044No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC34060.10807068444661051No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA34060.10807068444661051No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT33820.1073091763941388No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG33630.10670631585259868No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA33320.10572270128482272No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT32700.1037554721492708No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG32120.10191516102246417No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACC100.0070910677145.003512
ACGACGG100.00709174144.99892145
GAGCAGA6600.0107.707091
GTAACAA45200.0106.022434
GAAACAC14400.096.1624764
AAACACG15450.089.627175
CTATAGT259.388918E-486.9993444
TAACAAG59850.080.191545
AACACGT4400.079.090326
ACACGTG4650.074.838157
TAGTAAC65150.074.783082
AGCACGC502.3003304E-672.537431
GCACGCA502.3070625E-672.5017552
AGCGCAC300.001936188172.49945145
GCGGAGG300.001936188172.499454
AACACGA14600.071.50636
AGTAACA76350.064.000833
AGAAGCG5450.063.852735
CACGAGC16050.062.787698
CGGTGCA350.003566873562.1443632
Produced by FastQC (version 0.11.5)