FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_110_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_110_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6135362
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA67590.1101646488014888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGA38000.068.682186
ACGAGCG2200.065.907149
ACACGAG40800.063.613327
AAACACG60650.061.9190255
TAGTAAC115150.061.7033232
AACACGT19450.061.1293036
CACGAGC42600.060.5850648
GTAGTAA121050.059.5070041
ACAACGC24150.055.8368648
GTAACAA137650.050.9828764
CAACGCA29400.050.7978259
AGTAACA142400.049.740463
AACAACG27300.049.1285867
CGGAGCG3100.049.1114545
ACGAGCT11850.046.4965179
TAACAAG154500.045.4695325
ACGAGCC17800.045.2093439
GAAACAC86650.043.841754
TAGAAAC128500.043.0494732
ACGTGCA16050.039.7496079
Produced by FastQC (version 0.11.5)