FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_10_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_10_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463337
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT28890.197425473421365No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT26330.1799312120174642No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA25070.17132075523273177No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC24690.16872395080559024No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA23800.16264196148939034No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT22870.1562866243387545No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA22810.15587660258710057No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG20590.14070579777590533No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19910.13605888459049417No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC19180.13107028661203812No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT18860.12888350393655051No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC18740.12806346043324265No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT17790.12157144936538883No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC17510.11965801452433718No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC17360.11863296014520237No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG16910.11555779700779795No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGCTCTTCCGTGACC16850.115147775256144No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG16780.11466941654588109No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT16640.11371269912535525No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT16450.1124142969117845No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA16300.11138924253264969No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC16160.11043252511212386No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG16060.10974915552603398No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT15610.10667399238862955No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA15610.10667399238862955No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA15490.10585394888532171No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA15370.10503390538201385No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC15270.10435053579592396No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT15170.10366716620983411No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA14890.10175373136878244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA21200.0108.406394
GAAACAC7200.0102.706794
AAACACG7200.0102.706795
AACACGT2000.0101.4984746
AGCACGC700.093.273441
TAACAAG25250.091.018435
ACACGTG2250.090.2208567
AACACGA6350.084.4869166
ACGAGGA353.4380675E-582.855899
ACACGAG6600.081.286667
CAACGCA1750.078.71319
GCACGCA851.8189894E-1276.7661742
GAAGCGG3600.070.530841
TAGTAAC33500.069.904332
CACGAGC7650.066.338878
CACGCAC1009.094947E-1265.249023
CACGAGG451.19413155E-464.4434748
AGTAACA36500.063.759313
GTTAATA350.003557881662.182291
GAGCAGA3650.061.6144181
Produced by FastQC (version 0.11.5)