FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_109_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_109_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3019829
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC40550.087.429082
GTAGTAA43850.081.548621
AAACACG32000.071.5925455
AGCACGC13400.069.285841
GCACGCA14800.062.702692
AACACGA25700.061.7791186
AGTAACA58900.059.3283773
CACGAGC28600.053.9938478
ACACGAG29750.053.6125457
CGCACTT15700.052.1806535
GTAACAA67500.051.76954
TAACAAG68500.051.119585
ACGAGCA12300.049.511369
AACACGT15200.049.127476
ACGCACT21150.047.6469574
GAAGCGG17600.044.0971371
ACGAGCT11300.043.6275839
GAAACAC53200.043.1994634
ACAACGC12800.042.479768
GTAGAAA75450.041.6263161
Produced by FastQC (version 0.11.5)