FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_106_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_106_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672467
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT42360.253278540025005No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC32700.1955195528521639No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT28380.16968944678729087No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA26440.15808981582297288No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG25100.1500776995898873No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24790.14822415031208389No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT23380.13979349069368782No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT21520.1286721950268675No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19580.11707256406254953No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC18910.11306650594600672No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC17770.10625022795666521No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG17330.10361938382042815No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC17050.10194521027918636No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG16970.10146687498168871No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCCG100.0070909755145.00212145
GCACGCA6800.091.689772
GTAACAA46900.091.048714
TAGTAAC51700.083.717292
CACGCAC7550.082.581523
TAACAAG52300.081.647895
AGCACGC7750.079.54821
AGTAACA59150.073.295583
AAACACG17650.072.704275
AACACGA14050.069.660866
CGCACTT9650.068.366835
ACGCACT9950.067.7627944
ACACGAG14650.066.807857
ACAAGAG70150.060.768857
GTAGTAA74150.059.3732381
GAAACAC21550.059.2102364
CACGAGC16650.058.7828878
AACACGT5600.056.9634176
CAAGAGC53700.054.542938
CGGAGCA7600.053.420242
Produced by FastQC (version 0.11.5)