FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_105_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_105_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280630
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT45670.35662135042908566No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA33350.26041870017100954No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC30570.23871063460952813No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC28680.22395227349039146No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA26000.2030250735965892No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT23320.1820978737027869No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT23280.18178552743571524No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT22830.17827163193115889No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT22800.17803737223085514No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG22190.17327409165801208No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT22160.1730398319577083No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG21230.16577778124829187No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA20930.1634351842452543No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA20660.16132684694252047No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT19980.15601696040230198No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT19810.15468948876724736No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA19100.14914534252672512No Hit
ATCCAATGATGACCAATAACCCCATATACATCTTCGGAGCTTATGTGTAC18310.14297650375205953No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA17650.1378227903453769No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA17030.1329814232057659No Hit
ATATAAATCCTTACTTTCTCTTCATAGATGTACCCATACAGGCAGCAATT16690.13032647993565669No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC16160.12618789189695695No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA16110.12579745906311737No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA16040.12525085309574194No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC15850.12376720832715149No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT15610.12189313072472144No Hit
ATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCAT14510.11330360838025033No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT13860.10822798154033561No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT13740.10729094273912058No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA13700.10697859647204892No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC13630.10643199050467347No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA13610.10627581737113764No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG13240.10338661440072465No Hit
ATCTGGGACTCATAGCTGGAGAACTAAAAGGAACAGATCTATACTAAATA13050.10190296963213417No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG12970.10127827709799082No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC12960.10120019053122291No Hit
GATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACTGGTGT12950.10112210396445499No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACTGGTGTTCCCCC12830.10018506516323997No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG100.007088508145.01768145
AGCACGC3100.0117.013721
GCACGCA3200.0115.547432
CACGCAC3250.0113.7653353
AAACACG8750.096.1115
GAAACAC8950.093.9632644
GTAACAA29000.092.7468264
AAGAGCC7950.087.5441749
GAGCAGA5000.084.156271
TCTCGTT353.435566E-582.867256145
AACACGT2650.079.336916
TAACAAG34100.078.87565
TAGTAAC35550.076.68112
AGTAACA36900.073.872813
ACAACGC2800.072.497528
CAACGCA2800.072.497529
GCGAAGC300.001936129372.497523
AACACGA8850.071.268756
CGCACTT5700.069.953745
GTAGTAA40250.069.934961
Produced by FastQC (version 0.11.5)