FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_102_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_102_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4424163
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC17350.084.876651
TAGTAAC82750.075.873692
AAACACG47650.073.0314255
GTAGTAA88700.071.80631
GTAACAA90250.068.8438344
GCACGCA22200.066.622122
ACACGAG38950.060.3071757
AACACGA40250.059.2599756
CACGAGC38550.058.6761558
AACACGT19200.057.7725646
AGTAACA110350.056.6982843
CGCACTT25100.055.1684885
ACGAGCA25450.054.6946539
TAACAAG117050.053.2670255
CACGCAC28000.053.0795563
ACGAGCC11000.050.090159
ACGCACT35000.049.5063934
GAAACAC70050.049.160574
ACGAGCG1650.048.3326039
ACAACGC20900.047.176328
Produced by FastQC (version 0.11.5)