FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_100_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_100_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109484
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC41460.3736872275760624No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT40170.3620602009582833No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT30840.2779670549552765No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT24410.22001218584495136No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC23510.21190030680929153No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT19520.175937643084533No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC18780.16926787587743491No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC16980.1530441178061153No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC15450.1392539234454936No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC15020.13537824790623387No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT14920.1344769280133828No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC14880.13411640005624234No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC14760.13303481618482105No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC14260.1285282167205656No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG14240.12834795274199537No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT14100.12708610489200384No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT13900.12528346510630167No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA13810.1244722772027357No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA13630.12284990139560371No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT13550.12212884548132286No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG13370.12050646967419087No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG13140.11843343392063338No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA13130.11834330193134827No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA13120.11825316994206315No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG12700.11446762639208856No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA12640.11392683445637791No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA12540.11302551456352683No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC12410.11185379870282042No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA11980.10797812316356072No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT11850.10680640730285429No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT11790.10626561536714364No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT11750.1059050874100032No Hit
GAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAA11610.10464323956001167No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA11400.10275046778502439No Hit
CAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAA11250.10139848794574774No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAC100.007091077144.99924145
GAAACAC6850.0118.539524
GTAACAA21200.0116.614954
AGCACGC2600.0108.7984541
CGGTGCA203.8662503E-4108.749432
GCACGCA2650.0106.697552
AAACACG7800.0104.102015
CACGCAC2800.0100.9816063
TAACAAG26300.094.5527345
CGCACTT3400.087.426015
ACGCACT3450.086.1589664
AACACGT2200.085.6813666
AACACGA8200.076.036196
GAGCAGA2200.075.829221
TAGTAAC33300.075.5476462
TAGAAAC11750.074.042162
GAAGCGG3400.072.532311
ACGAGGA300.001935839172.499624
CGGAGCA6600.071.401142
ACACGAG9000.069.277417
Produced by FastQC (version 0.11.5)